Endo-Glucanase Producing Thermophillic Bacillus subtilis: Gene Isolation and Structure Function Prediction

Beslin Joshi J; Priyadharshini R; Sivakumar Uthandi

doi:https://doi.org/10.29321/MAJ.S.000451

Research Article | Open Access | Peer Review

Endo-Glucanase Producing Thermophillic Bacillus subtilis: Gene Isolation and Structure Function Prediction

Beslin Joshi J Priyadharshini R Sivakumar Uthandi

Volume : 107

Issue: Special

Pages: 25 - 26

DOI: https://doi.org/10.29321/MAJ.S.000451

Downloads: 0

Published: December 31, 2020

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Abstract
Keywords
Introduction
Methodology
Results and Discussion
Conclusion

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Abstract

Cellulase producing thermophillic bacteria, Bacillus subtilis VSDB5 was isolated from biotrap based enrichment at hot springs in Himachal Pradesh. Molecular confirmation for the presence of cellulase in B. licheniformis using spe- cific primers amplified partial gene fragment around 1300 bp. Cellulase gene specific PCR followed by sequencing and BLAST analysis revealed the fragment had 98% homology to Endoglucanase gene of Bacillus subtilis 168. Mul- tiple alignment and homology modelling revealed it belongs to GH5 endoglucanase with its structure containing a classical (Î²/Î±)8-barrel fold with conserved active site residues, Glu257 and Glu165.

DOI

https://doi.org/10.29321/MAJ.S.000451

Pages

25 - 26

Creative Commons

Copyright

© The Author(s), 2025. Published by Madras Agricultural Students' Union in Madras Agricultural Journal (MAJ). This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited by the user.

Keywords

Cellulase hydrolysis Endoglucanase Bacillus subtilis cloning

Abstract
Keywords
Introduction
Methodology
Results and Discussion
Conclusion

Endo-Glucanase Producing Thermophillic Bacillus subtilis: Gene Isolation and Structure Function Prediction

Abstract

Keywords

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Endo-Glucanase Producing Thermophillic Bacillus subtilis: Gene Isolation and Structure Function Prediction

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ISSN : 0024-9602

eISSN : 2582-5321

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