Different methods of inoculations were tried for proving Koch’s postulates. Among them, the pin prick plus spore suspension spray was found be the best method under glasshouse. Out of the ten isolates of Colletotrichum gloeosporioides, isolate C1 was highly virulent and C7 was avirulent. The virulent isolate of C. gloeosporioides produced more cellulolytic enzymes invitro than the avirulent one and the activity of these enzymes increased with the increase in age of culture. Furthermore, the virulent isolate of C. gloeosporioides produced more pectinolytic enzymes than the avirulent one. All the pectinolytic enzymes produced by virulent isolate were highly active in 10-day-old culture and the activities decreased with the increase in age of culture. On the contrary, the activity of enzymes produced by avirulent isolate of pathogens did not decrease and these enzyme activities increased with the increase in the age of culture.
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