Abstract

Studies were conducted to extract and evaluate the intracellular bioactive molecules of Ophiocordyceps sinensis against plant pathogens. Methanolic fraction of mycelium borne bioactive molecules at 200 μL concentration showed the maximum mycelial inhibition of Fusarium oxysporum f. sp. cubense (48.67 per cent) and F. o. f. sp. lycopersici (46.47 per cent), which cause wilt disease in banana and tomato, respectively. GC-MS analysis of methanolic fraction of mycelial mat extract indicated the presence of 14 different compounds hydroxylamine; glycerin; oxime-,methoxy-phenyl; adipamide; 1,2,3-Benzenetriol; cyclododecane; imidazole-5-carboxylic acid; 1,3,5-Benzenetriol; 1,3-Cyclohexanedione, 2-propyl; 2-Propenoic acid, tridecyl ester; n-Decanoic acid; 5-Undecene, 9-methyl; L-Ornithine, N5-(aminocarbonyl) and pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro. Among these, the compound hydroxylamine identified at 1.817 RT exhibited the maximum peak area of 35.00 per cent. This compound is also known to possess important pharmacological and antimicrobial properties.

Keywords: O. sinensis, Antifungal bio-molecules, TLC and GC-MS