Madras Agricultural Journal
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Research Article | Open Access | Peer Review

Complete RNA1 Genome Sequence of Tobacco streak virus Infecting Okra (Abelmoschus esculentus L.) and Its Phylogenetic Relationship

Volume : 103
Issue: Jul-sep
Pages: 254 - 257
Published: April 30, 2023
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Abstract


Tobacco streak virus (TSV) was detected from infected okra (Abelmoschus esculentus L.) samples collected from the field. They were also serologically positive in direct antigen coating enzyme linked immunosorbant assay (DAC-ELISA). Sap inoculation of the okra strain induced local as well systemic infection with the production of circular necrotic lesions infection on cowpea cv. C 152 plants. TSV was characterized by RT-PCR using replicase gene specific primers with three different fragments. The replicase gene was amplified into three different fragments with a size of 1.11, 1.19 and 1.26 kb including the UTR region as part of RNA1 of TSV. The BLAST analysis of full length replicase gene had nucleotide similarity of 99.5 to 79.0 % and amino acid sequence shared sequence similarities of 99.4 to 86.8 % with known strains of TSV. Phylogenetic analysis of the nucleotide confirms that the okra strain of TSV forms single subgroup with other crop of Indian isolates.

DOI
Pages
254 - 257
Creative Commons
Copyright
© The Author(s), 2025. Published by Madras Agricultural Students' Union in Madras Agricultural Journal (MAJ). This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited by the user.

Keywords


Replicase gene Tobacco streak virus Sequence analysis.
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