Isolation of Chlorpyriphos and Carbofuran Degrading Bacteria from Pre-treated Soils
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Author:Nafeesa M*, Ramaraju K, Kuttalam S and Doraisamy P
p-ISSN:0024-9602, e-ISSN:2582-5321, Vol:103, Issue:oct-dec
DOI: https://doi.org/10.29321/MAJ.10.001048
Abstract
Investigations were carried out at Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore to isolate chlorpyriphos and carbofuran degrading bacterial isolates from pre-treated soils. Five each of chlorpyriphos and carbofuran degrading bacterial isolates were obtained from the enrichment cultures. Chlorpyriphos degrading bacterial isolates viz., CPY-1, CPY-2, CPY-3 and CPY-4 were tentatively identified as Serretia sp., Pseudomonas sp., Klebsilla sp. and Acinetobacter sp., respectively. Carbofuran degrading bacterial isolates viz., CF-1, CF-2 and CF-7 were tentatively identified as Enterobacter sp., Bacillus sp. and Bacillus sp., respectively. The identity of CPY-5, CF-3 and CF-4 was not confirmed. Based on nucleotide homology and phylogenetic analysis the potential isolates CF-7 and CPY-4 were confirmed as Bacillus subtilis and Acinetobacter sp. respectively.
Key words : Chlorpyriphos, Carbofuran, Bacterial isolates, Enrichment culturing, Isolation