Abstract

Investigations were carried out at Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore to isolate chlorpyriphos and carbofuran degrading bacterial isolates from pre-treated soils. Five each of chlorpyriphos and carbofuran degrading bacterial isolates were obtained from the enrichment cultures. Chlorpyriphos degrading bacterial isolates viz., CPY-1, CPY-2, CPY-3 and CPY-4 were tentatively identified as Serretia sp., Pseudomonas sp., Klebsilla sp. and Acinetobacter sp., respectively. Carbofuran degrading bacterial isolates viz., CF-1, CF-2 and CF-7 were tentatively identified as Enterobacter sp., Bacillus sp. and Bacillus sp., respectively. The identity of CPY-5, CF-3 and CF-4 was not confirmed. Based on nucleotide homology and phylogenetic analysis the potential isolates CF-7 and CPY-4 were confirmed as Bacillus subtilis and Acinetobacter sp. respectively.

Keywords: Chlorpyriphos, Carbofuran, Bacterial isolates, Enrichment culturing, Isolation