Sugarcane Bagasse as Substrate for Ethanol Production

Dharmaraj V*; Venkatachalam P and Gopal N O

doi:https//doi.org/10.29321/MAJ.10.001160

Madras Agricultural Journal

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Sugarcane Bagasse as Substrate for Ethanol Production

Author: Dharmaraj V*, Venkatachalam P and Gopal N O,

p-ISSN: 0024-9602, e-ISSN:2582-5321, Vol: 101, Issue: jan-mar,

DOI:https//doi.org/10.29321/MAJ.10.001160

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Abstract

Pre treatment of sugarcane bagasse with 2% sodium hydroxide at 1.40 kg cm-2 for 1 h resulted in 83% delignification. The hydrolysis of alkali treated bagasse with a commercial preparation of cellulase for 2 h at 50Â°C resulted in release of 65% total reducing sugars. Maximum sugars were released at enzyme loading of 1.5% (v/v) .The fermentation of hydrolysate supplemented with nutrients by S. cerevisiae resulted in the production of 20-30 g l-1 ethanol after 48 h of incubation, which was further improved with addition of yeast nitrogen base and inoculated

Keywords: Cellulose, Ethanol, Fermentation, Sugarcane bagasse, S. cerevisiae

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