In vitro response of explant size and age on multiple shoot induction from shoot tips of castor (Ricinus communis L.)

B. Rampal and Ramesh Thatikunta

doi:https://doi.org/10.29321/MAJ.10.100727

Madras Agricultural Journal

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In vitro response of explant size and age on multiple shoot induction from shoot tips of castor (Ricinus communis L.)

Author: B. Rampal and Ramesh Thatikunta,

p-ISSN: 0024-9602, e-ISSN:2582-5321, Vol: 93, Issue: jan-jun,

DOI:https://doi.org/10.29321/MAJ.10.100727

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Abstract

The response of shoot tips to initiate multiple shoots was studied in two genotypes of castor GCH 4 and Kranthi. In vitro shoot bud induction response varied with the explant size and age. Five mm shoot tips or shoot tips obtained from nine day old seedlings initiated a maximum of 4.6 and 5.6 shoot buds per explant on MS + BAP 5.0 mg/1 respectively. Elongated shoots were induced for rooting on MS +IBA 0.1 mg/1. Complete plants were hardened and later transferred to pots and grown up to maturity of primary spikes.

Keywords: Multiple shoot bud induction, Ricimus communis

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