Abstract

Shoot buds of shoot tip and nodal stem cutting of Morus alba were cultured on MS medium to produce multiple plantlets. Higher rate of shoot induction, multiplication and plantlet regeneration were observed on MS medium with exogenous addition of growth hormones viz. BAP (1.5 mg l-1) and IBA (0.5 mg l-1). Plantlets on transfer to rooting medium of ½ MS with IBA (1.0 mg l-1) produced vigorous rooting. From single shoot bud of mulberry plant about 225 plantlets within 70-75 days were obtained. Successful transfer and survival of 90-95 percent of regenerants to soil have been accomplished. Therefore, with high multiplication rate, present study provides the scope for rapid clonal multiplication of a superior clone of M. alba.

Keywords: Mulberry, shoot bud, apical bud, axillary bud, exogenous growth hormones, shoot induction, root induction, multiplication