Influences of nutrition, pH and temperature requirements on the mycelial growth of black poplar mushroom Agrocybe aegerita were studied in vitro. Good mycelial biomass was obtained at 25°C and pH of 7. Upon six carbon and nine nitrogen sources tested, sucrose and peptone proved the best carbon and nitrogen respectively. The best carbon and nitrogen sources at 3:3 ratio of sucrose: peptone was found to increase the mycelial biomass in liquid medium.
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