Two single nucleotide polymorphic mutations and deletion affectingY1059V1060V1061 amino acid residues in a host translation initiation factorfour gamma (eIF4G) gene in rice are reported to confer resistance to ricetungrospherical virus in resistant genotypes. A CRISPR-based adenine baseediting vector was used to target these residues in a susceptible indicacultivar, ASD16.Agrobacterium-mediated transformation of ASD16 generated16 missense mutants and two deletion mutants. Substitution mutationsoccurred at A5> G5 and A4> G4, where 5.5 % and 3.37 % of adenosines gotconverted to guanosines, respectively. The mutantsgenerated had missensemutations affecting the YVV residues and the residues immediately adjacentto YVV.Thus,these novel mutationsare promising candidates in impartingresistance against rice tungro disease.
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