Production of Volatile Organic Compounds of Fusarium oxysporum f. sp. lycopersici on Coinoculation with the Metabolites of Chaetomium globosum

Abstract

he present study focused on divulging the effect of 15 and 30 days oldculture filtrates of Chaetomium globosum on the mycelial growth and toxinproduction of Fusarium oxysporum f. sp. lycopersici. The cell-free culture (CFC)filtrate of C. globosum was inoculated in the liquid medium of F. oxysporum f.sp. lycopersici. The results revealed that mycelial growth was reduced in theCFC filtrate of C. globosum inoculated medium compared to control (pathogenalone). The mycelial dry weight of the F. oxysporum f. sp. lycopersici was0.864g in fifteen days old CFC filtrate followed by 30 days old CFC filtrate of C.globosum (1.374g) amended medium. Metabolites from the CFC filtrate andcontrol were extracted separately using chloroform followed by ethyl acetateand the extract was subjected to GC-MS analysis. GC-MS analyses showedthat methyl ester group was present in 15 days old culture filtrate condensatecompared to 30 days old CFC. The compounds are methyl tetradecanoate,octadecanoic acid, methyl ester, and DL-Proline, 5-oxo-, methyl ester.Thirty days old culture filtrate condensate of C. globosum contained morephenol groups like phenol 2,4-bis (1,1-dimethylethyl) at 17.62 RT followedby diisooctyl phthalate, 17-pentatriacontene, cholestan-3-ol, 2-methylene-,(3a,5a) and bicycle [4.1.0]heptanes,-3-cyclopropyl,-7-hydroxymethyl,trans.Metabolites of F. o. f. sp. lycopersici were also characterized by GC-MS,which showed very few volatile compounds like methyl tetradecanone , oleicacid, eicosyl ester, methyl stearate, and bis(2-ethylhexyl)phthalate. Thesecompounds were not detected in co-inoculation of 15 and 30 days old CFCfiltrate of C. globosum and F. o. f. sp. lycopersici. The difference in thevolatile profile may be due to the effect of the metabolites of C. globosumon F. o. f.sp. lycopersici.

DOI

https://doi.org/10.29321/MAJ.10.000508

Pages

259 - 265

Creative Commons

Copyright

© The Author(s), 2025. Published by Madras Agricultural Students' Union in Madras Agricultural Journal (MAJ). This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited by the user.

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