Characterization of Novel Cellulosome Complex of Clostridium cellulovorans TCW from Coffee Pulp Waste

Sugitha T C K; Ramasamy K; Doraisamy P; Sivakumar U; Maheswari M

doi:https://doi.org/10.29321/MAJ.S.000449

Research Article | Open Access | Peer Review

Characterization of Novel Cellulosome Complex of Clostridium cellulovorans TCW from Coffee Pulp Waste

Sugitha T C K Ramasamy K Doraisamy P Sivakumar U Maheswari M

Volume : 107

Issue: Special

Pages: 20 - 21

DOI: https://doi.org/10.29321/MAJ.S.000449

Downloads: 0

Published: December 31, 2020

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Abstract
Keywords
Introduction
Methodology
Results and Discussion
Conclusion

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Abstract

In this present investigation a new bacterial strain Clostridium cellulovorans TCW 3 was obtained from coffee pulp waste enriched with goat rumen fluid. The enzyme production profile showed cellulase and xylanase activities when grown on coffee pulp in different incubation periods. Cellulase and xyalnase activity were recorded max- imum at 60oC and pH 7.5 for cellulase. Endoglucanase , exoglunase and xylanse attained stable at 10- 12 hours where as xyalanse was stable after 6 hours of incubation. The apparent km and Vmax values are maximum for soluble substrates like crystalline cellulose and soluble xylan (7.5 and 1.25 mg/ml respectively). This opens a new paradign for using coffee pulp waste as a biomass for deriving biofuels and other green chemicals like chlorogenic acid, caffeine, pectin, sugars etc

DOI

https://doi.org/10.29321/MAJ.S.000449

Pages

20 - 21

Creative Commons

Copyright

© The Author(s), 2025. Published by Madras Agricultural Students' Union in Madras Agricultural Journal (MAJ). This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited by the user.

Keywords

Coffee pulp waste Xylanase Cellulase Kinetic parameters

Abstract
Keywords
Introduction
Methodology
Results and Discussion
Conclusion

Characterization of Novel Cellulosome Complex of Clostridium cellulovorans TCW from Coffee Pulp Waste

Abstract

Keywords

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Characterization of Novel Cellulosome Complex of Clostridium cellulovorans TCW from Coffee Pulp Waste

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eISSN : 2582-5321

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