Abstract

A simple and rapid method for the analysis of flubendiamide and its major metabolite desiodo flubendiamide, in soils and water with acetonitrile and ethyl acetate, respectively, is presented. Quantification was performed by reversed phase HPLC with UV detector. Mean recoveries of flubendiamide and desiodo flubendiamide in soils ranged from 88.01- 90.26 % and 90.5-92.29 % respectively. The applicability of the method to the determination of flubendiamide and desiodo flubendiamide in spiked water at three pH levels (4, 7 and 9.2) was evaluated. Recoveries for flubendiamide and desiodo flubendiamide spiked water were in the ranges 89.21-92.32 % and 92.2-95.57 % respectively. The detector linearity and the repeatability of the method were very precise.

Keywords: Flubendiamide, Desiodoflubendiamide, HPLC-UV, Soil, Water.