Abstract

The current investigation was aimed to develop mutants with respect to pollen / spikelet sterility and good floral traits for use in two-line breeding. Two TGMS lines viz., TS 6 and CBTS 0282 were subjected to mutagenesis with gamma rays (300 and 350 Gy) and Ethyl methane sulphonate (0.5 percent and 0.6 percent) for developing new TGMS lines with desirable floral traits. The highest mean level of gain of function for well exerted stigma over check (IR 58025A) were observed in CBTS 0282 - 0.5 per cent EMS treated 27-4-1-7-4 (124.7 percent), TS 6 - 0.6 per cent EMS 196-1-3-6-3 (122.21 percent), TS 6 - 0.6 per cent EMS 196-1-3-4-1 (120.97 percent) and CBTS 0282 - 0.5 per cent EMS 27-4-1-3-3 (120.94 percent). These mutant populations also showed significant gain of function for days to 50 percent flowering, pollen sterility percentage and spikelet sterility percentage. Hence identified new lines are very much useful for using as parental lines in future two line breeding programme.

Keywords: TGMS lines, mutagenesis, mutants, EMS, pollen and spikelet sterility