Abstract

n the present study, 30 accessions of chickpea were analyzed for total seed protein profile using Sodium Dodecyl Sulphate-Poly Acraylamide Gel Electrophoresis (SDS-PAGE) to ascertain the extent of genetic variation. Based on dendrogram of SDS- PAGE, genotypes were divided into nine clusters taking 75% similarity as standard. From the clustering pattern of the 30 genotypes, it was clear that cluster III evolved as large cluster comprising 12 genotypes and clusters I, II, VI, VII, VIII and IX comprised single genotype each. Cluster IV and V comprised two and ten genotypes, respectively. The minimum similarity coefficient was 0.11 hence the genotypes, PG 061 and PG 059 were most dissimilar with PG041. It was observed that four genotypes namely PG 047, PG 052, PG 058 and PG 057 had shown similarity coefficient 1.0. Therefore, these genotypes may be considered genetically similar. SDS-PAGE can be used for characterization of cultivars and species, establishment of phylogenetic relationship among taxa and for genetic studies in many crops

Keywords: Chickpea, SDS-PAGE, total seed protein, similarity coefficient