Madras Agricultural Journal
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Seed Viability Test with 2, 3, 5 Tri-Phenyl Tetrazolium Chloride

Abstract

In 1941 Kuhn and Jerchel (6) drew attention to the properties of colourless tetrazolium salts which get reduced by some phytochemical process to stable red formosans on contact with the tissues of embryos of viable seeds and suggested their use as reduction indicators of living tissues. Since then, the test has been in use in German Breeding Stations for testing viability in oats.

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