Response Surface Methodology (RSM) was employed to optimize acid pretreatment condition for maximum glucose yield of rice straw with respect to H2SO4 concentration (1.0-5.0%), reaction temperature (60-100°C) and pretreatment time (30-90 min). The maximum glucose yield of 259.40g kg-1 of biomass was obtained at the desirable pretreatment condition of H2SO4 3 % at 80°C for 60min. The ANOVA test revealed that the model and all independent parameters were statistically significant at 95% confidence level.
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