Abstract

Pulses are the important source of protein in vegetarian diet and they are affected by several viral diseases. Among the virus diseases, yellow mosaic disease (YMD) caused by Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus (family Geminiviridae) is a serious malady in cultivation of grain legumes in India and cause severe yield loss. YMD has very narrow host range within legumes and cause biologically indistinguishable symptoms, so the identification of virus is difficult. The molecular detection of MYMV from infected blackgram plants showing yellow mosaic symptoms was carried out. PCR with begomovirus specific primers detected the presence of begomovirus from the infected samples with amplicons of ~1100bp. The PCR with MYMV-specific coat protein primers detected the presence of MYMV from the samples collected from three districts of Tamil Nadu with the amplicons of ~1000bp. The dot blot hybridization showed positive response with the infected samples and negative response with healthy samples. The associated satellite DNA components were detected using universal abutting primers. Hence, molecular detection of MYMV seems simple and very sensitive method.

Keywords: Mungbean Yellow Mosaic Virus, Polymerase Chain Reaction, Dot blot hybridization