The frequency of chlorophyll mutations as number of mutations per 100 M; spikes was as efficient as the number of mutants per 100 M. plants. The relationship between dose and frequency remained the same irrespective of the method of estimation of mutation frequencies. The frequencies decreased at high doses even when estimated on My plant basis. The frequency of chlorophyll mutation in gamma irradiation increased upto the middle dose and thereafter followed an inconsistent trend whereas. in EMS treatments the frequency increased with increase in dosage. The rate of mutation was higher in primary spikes than the lust and second formed spikes. Induced chlorophyll mutation spectrum differed between gamma rays and EMS. Albing was the most fre quent type following irradiation while xantha was predominert with EMS treatments.
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Tamil Nadu Agricultural University
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Tamil Nadu, India.
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