Three greengram cultures viz. TT9-E, DT 9-E and DT 12-E were treated with 0.25% EMS for one hour under reduced pressure, after different periods of pre-soakings. The mutation frequency was recorded in Me and My generations in selfed and unselfed progenies. The peak of mutation frequency occurred in the treatment after 12.30 hours. of pre-soaking in the M, generation. The selfed progenies had higher mutation frequ- encies than unsalfed progenies. The lower mutation frequencies in unselfed progenies Indicated definite occurrence of certain amount of outcrossing in the M, generation due to induction of M, pollen sterility. Even in a self pollinated crop like greengram upto 2.63% mutants could not get expression due to induction of pollen sterility and outcrossing.
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