Abstract

Effect of basal media and their strength, growth hormone composition and concentration and different explants on callus induction, proliferation and induction of embryogenesis were examined in Vitex negundo L. Sixteen media composition analysis of callus induction frequency and thirty two combinations for regeneration response were examined. Four explants viz., leaf, stem, shoot-tip and bark were tried. Basal medium MS was found best for callus induction and regeneration in its full strength. For callus induction MS +2,4-D, (2-2.5 mg 1-1) + Kn (0.5-0.75 mg 1-1) and for embryoid formation MS + BAP (1.5 mg 1-1) + IAA (0.5 -0.75 mg 1-1) were found efficient. Among explants, leaf explant produced highest callus induction and proliferation response followed by stem and shoot-tip while bark explants responded poorly

Keywords: Nochi, callus induction, proliferation, embryogenic calli, growth regulators