Effect of basal media and their strength, growth hormone composition and concentration and different explants on callus induction, proliferation and induction of embryogenesis were examined in Vitex negundo L. Sixteen media composition analysis of callus induction frequency and thirty two combinations for regeneration response were examined. Four explants viz., leaf, stem, shoot-tip and bark were tried. Basal medium MS was found best for callus induction and regeneration in its full strength. For callus induction MS +2,4-D, (2-2.5 mg 1-1) + Kn (0.5-0.75 mg 1-1) and for embryoid formation MS + BAP (1.5 mg 1-1) + IAA (0.5 -0.75 mg 1-1) were found efficient. Among explants, leaf explant produced highest callus induction and proliferation response followed by stem and shoot-tip while bark explants responded poorly
For MASU related issues :
PUBLISHER
SECRETARY
Madras
Agricultural Students Union
Tamil Nadu Agricultural University
Coimbatore - 641 003
Tamil Nadu, India.
For MAJ related issues :
EDITOR-IN-CHIEF
Madras Agricultural Journal (MAJ)
Madras
Agricultural Students Union
Tamil Nadu Agricultural University
Coimbatore - 641 003
Tamil Nadu, India.
Copyright © Madras Agricultural Journal | Masu Journal All rights reserved.
Please provide the registered email ID
Please enter the new password
