INITIATION OF CALLUS AND PLANT REGENERATION IN COTTON (Gossypium hirsutum L.)

A.KALAMANI

doi:https://doi.org/10.29321/MAJ.10.A01585

Madras Agricultural Journal

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INITIATION OF CALLUS AND PLANT REGENERATION IN COTTON (Gossypium hirsutum L.)

Author: A.KALAMANI,

p-ISSN: 0024-9602, e-ISSN:2582-5321, Vol: 81, Issue: nov-nov,

DOI:https://doi.org/10.29321/MAJ.10.A01585

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Abstract

The hypocotyl explants of MDU 9 cultured on Murashige and Skoog medium supplemented with kinetin (1.0 mg/lit.)+ IAA (1.5 mg/lit.) formed callus in 20 days. The calli on sub culture to media containing benzyl amino purine (2.0 mg/lit.) developed shoots. Multiple shoots were formed from each callus mass. These on further sub culture with reduced level of benzyl aminopurine developed well. Root initiation and growth was promoted by lowering the sucrose concentration and supplementing the culture media with NAA (3.0 mg/lit.).

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