Abstract

The minimum acquisition feeding and inoculation feeding period of vector A. marvae were found to be 30s and 1 min respectively. But the percentage of transmission was maximum when an acquisition feeding period of 30 min inoculation feeding period of 2 h were given. Influence of starvation before acquisition and inoculation feeding period proved that pre-acquisition starvation for 2 h produced maximum infection but post-acquisition starvation decreased the percent infection. The vector could retain the virus for 1 h only. A single aphid could transmit the virus to healthy test plants, but maximum percentage of transmission was obtained with 10 aphids.

Keywords: Acquisition threshold. Inoculation threshold, pre- aquisition fasting, post-acquisition fasting.