The in vitro response of sugarcane varieties CO 86249 and CO 8021 was assessed on twelve media for callus induction, multiplication, shooting and rooting. CO 86249 recorded the highest callusing frequency at 2,4-D 3 mg/lit. with 0.1 mg/ lit casein hydrolysate followed by 2,4-D 3 mg/lit with 100 ml of coconut milk. CO 86249 had significant shoot multiplication ratio and length in MS media supplemented with1 mg/lit IBA+ 2.0 mg/lit+1.0 mg/lit kinetin. The half strength MS media supplemented with 1 mg/lit NAA+ 1.25 mg/lit IBA was promoted profuse root in both the varieties. Whereas, full strength MS media along with different levels of growth hormone showed reduction in number of roots in both the varieties.
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