Similarity Analysis of Fluorescent Pseudomonas
from Apple and Pear Rhizosphere
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Author:Sheetal Rana, Ranjna Sharma and Mohinder Kaur
p-ISSN:0024-9602, e-ISSN:2582-5321, Vol:101, Issue:oct-dec
DOI: https://doi.org/10.29321/MAJ.10.001221
Abstract
Molecular markers have been found useful in assessing the genetic diversity in a precise
manner, which is based on naturally occurring DNA polymorphism. Random Amplified
Polymorphic DNA analysis was performed using four arbitary decamer primers to observe
genetic homogeneity/ polymorphism among 10 isolates of fluorescent Pseudomonas spp.
isolated from apple and pear rhizosphere from different locations of Mandi and Chamba
districts. Out of 52 bands generated with 4 RAPD primers, 13.46% were monomorphic bands,
while 86.33% were polymorphic bands. The result showed that the isolates of fluorescent
Pseudomonas sp. were divided into 2 clusters by RAPD primers. Among all the combinations
maximum similarity (90.48%) was found between PN-7-Cha and PN-2-San isolates of Chamba
and Mandi districts. However, minimum similarity (36.7%) was observed in PN-11-San and PN-
7-Cha isolates of Mandi and Chamba districts. This technique is highly beneficial for testing
similarity analysis among fluorescent Pseudomonas spp. isolated from different locations.
Key words : PGPR, Fluorescent Pseudomonas, RAPD