Similarity Analysis of Fluorescent Pseudomonas from Apple and Pear Rhizosphere

Abstract

Molecular markers have been found useful in assessing the genetic diversity in a precise manner, which is based on naturally occurring DNA polymorphism. Random Amplified Polymorphic DNA analysis was performed using four arbitary decamer primers to observe genetic homogeneity/ polymorphism among 10 isolates of fluorescent Pseudomonas spp. isolated from apple and pear rhizosphere from different locations of Mandi and Chamba districts. Out of 52 bands generated with 4 RAPD primers, 13.46% were monomorphic bands, while 86.33% were polymorphic bands. The result showed that the isolates of fluorescent Pseudomonas sp. were divided into 2 clusters by RAPD primers. Among all the combinations maximum similarity (90.48%) was found between PN-7-Cha and PN-2-San isolates of Chamba and Mandi districts. However, minimum similarity (36.7%) was observed in PN-11-San and PN- 7-Cha isolates of Mandi and Chamba districts. This technique is highly beneficial for testing similarity analysis among fluorescent Pseudomonas spp. isolated from different locations.

DOI

https://doi.org/10.29321/MAJ.10.001221

Pages

386 - 389

Creative Commons

Copyright

© The Author(s), 2025. Published by Madras Agricultural Students' Union in Madras Agricultural Journal (MAJ). This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited by the user.

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