Phylogenetic Relationship of DpNPV (Diaphania pulverulentalis Nuclear Polyhedrosis Virus) with Other Lepidopteran NPVs based on Polyhedrin Gene 270
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Author:P. Priyadharshini, K.C. Narayanaswamy and C.A. Mahalingam
p-ISSN:0024-9602, e-ISSN:2582-5321, Vol:98, Issue:jul-sep
DOI: https://doi.org/10.29321/MAJ.10.100294
Abstract
The mulberry leaf-roller, Diaphania pulverulentalis (Hampson) is a major defoliator pest of
mulberry in sericultural tracts of Karnataka, India. A nuclear polyhedrosis virus isolated from
D. pulverulentalis larvae was analysed via its polyhedrin gene, which was amplified from
DpNPV DNA in polymerase chain reaction using degenerate set of primers and the sequence
was analysed phylogenetically with other polyhedrin genes. The analysis revealed that DpNPV
was closely related to Galleria mellonella NPV, Plutella maculipennis NPV and Autographa
californica NPV and Autographa biloba NPV and distinct from Bombyx mori NPV.
Key words : Diaphania pulverulentalis, nuclear polyhedrosis virus, polyhedrin gene, phylogenetic analysis.