Plant regeneration from scutellum- derived callus of Assam rice collection (Oryza sativa L.)

S. Chitra and C.R. Ananda kumar

doi:https://doi.org/10.29321/MAJ.10.100726

Madras Agricultural Journal

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Plant regeneration from scutellum- derived callus of Assam rice collection (Oryza sativa L.)

Author: S. Chitra and C.R. Ananda kumar,

p-ISSN: 0024-9602, e-ISSN:2582-5321, Vol: 93, Issue: jan-jun,

DOI:https://doi.org/10.29321/MAJ.10.100726

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Abstract

In vitro plant regeneration from callus induced from embryos of mature seeds of 8 ARC lines along with japonica line Taipei 309 were studied. Observations on callus induction were carried out on MS medium containing 2mg 2,4-D L-1. The callus induction frequency varied from 72.0% to 96% and regeneration frequency from 0 to 26.66% depending upon the genotype used. Of the nine lines tested regeneration were produced in considerable numbers in five lines only. (ARC 7140, ARC 12194, ARC 12451, ARC 14763, ARC 15759). This study regarding enhancement of somatic embryogenesis could be used for further genetic transformation studies.

Keywords: Assam Rice Collection (ARC), in vitro culture, callus

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