Methanogens are strictly anaerobic organisms that produce methane. Based on the nature of substrate utilization, they are classified into acetoclastic, hydrogenotrophic and methylotrophic methanogens. Methanothrix soehngenii is a methanogen believed to be acetoclastic. A strain RWL1, similar to M. soehngenii with hydrogenotrophic activity has been isolated, characterized and identified by 16S rRNA sequencing. The strain RWL1 utilizes H2+CO2 (4:1) and has recorded methane production of 74.82%, thereby opening a new gate for substrate diversity studies. This study reveals the hydrogenotrophic nature of strain RWL1 clustered with M. soehngenii..
Key words : Anaerobe, methanogen, aceticlastic, hydrogenotrophic, substrate.