Standardization of spore load for AMF inoculation and its effect on rice root colonization and root system architecture in vitro
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Author:Nivedha, R.M1, Marimuthu, P*1, Balachandar,D1 and Nakeeran, S2
Page No:1
DOI : https://doi.org/10.29321/MAJ 2019.000292
Abstract
Arbuscular Mycorrhizal Fungi (AMF) can form symbiotic relationship with
rice, preferably in the modern rice production systems such as System of
Rice Intensification (SRI), aerobic and upland conditions. Their association
with rice roots under in vitro conditions was investigated in this study.
A suspension containing one lakh (1, 00,000) spores of Rhizophagus
irregularis was employed to standardize optimum AMF spore load/seedling
and evaluate its effect on Root System Architecture (RSA). The results
indicated that different spore loads of AMF inoculated with rice seedlings,
under in vitro conditions, modified most of the traits of RSA of rice at early
stages significantly better than the uninoculated Control. The in vitro study
clearly showed that the seedlings of rice with AMF inoculated with a dose of
14 µl/seedling (31 spores/ seedling) recorded maximum root colonization
of 100 per cent by AMF in rice.
Key words : AM Fungi, Spore load, Rice (Oryza sativa), RSA, in vitro, Principal Component Analysis (PCA).
