MADRAS AGRICULTURAL JOURNAL

Cloning and In silico analysis of Casparian strip membrane domain protein (CASP) from rice

Abstract

                                Casparian strip (CS) seals the adjacent endodermal cells to form a tight junction and thereby regulate the movement of water and salts across the endodermis. Casparian strip membrane domain proteins (CASPs) play an important role in the formation of the CS. Among the CASP proteins, the role of CASP1 was much emphasized in the making of a protein scaffold on the surface of endodermal cells that inturn leads to the formation of CS. In this study, full-length cDNA of CASP1 was cloned from the roots of salt-stressed rice cultivar ASD16 using specific primers derived from the corresponding reference gene sequence in GenBank. The cDNA fragment of 813 bp obtained through RT-PCR was cloned in an intermediary cloning vector pJET1.2. The recombinant clone carrying CASP1 was characterized by restriction analysis and sequencing. Further, in silico analysis of CASP1 revealed that it is a transmembrane protein consisting of five transverse helices as per secondary and tertiary structure analysis. 3D structure of CASP1 protein was obtained by I TASSER online server. The structure was validated using PROCHECK structural analysis tool..

Key words : Radicle emergence, cluster bean, germination, vigour and field emergence

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

stress. With this background, the present investigation was carried out in CO 8 variety of greengram to study the physiological characters and yield responses of greengram under different elevated temperature stress conditions. The experiment was conducted in the open top chambers located in the Department of Crop Physiology, Tamil Nadu Agriculture University, Coimbatore during 2016-2017. Elevated temperature stress was given two levels, by raising the temperature up to 2ºC and 4ºC from the ambient temperature. Plants subjected to an elevated temperature at various stages viz., vegetative, flowering and pod development. Observations on various physiological parameters viz., leaf area, gas exchange parameters like photosynthetic rate, stomatal conductance, transpiration rate, leaf temperature were recorded. Also, osmotic potential and osmotic adjustments were studied under stress period. The response of yield was also recorded under different elevated temperature stress. Among the growth stages, the flowering stage was more critical to the elevated temperature and the impact of 4ºC raise from ambient temperature was higher when compared with the elevated temperature of 2ºC.

Key words : Leaf area, gas exchange parameters, osmotic adjustment and yield traits..

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

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  • Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.