MADRAS AGRICULTURAL JOURNAL

Studies on Extraction of Betalain Pigments by Different Solvents and Assessing Antioxidant Activity of Bougainvillea spectabilis and Celosia argentea Flowers

Abstract

                              Betalain is a natural pigment with antioxidant property which is used as a food colourant. Betalain was extracted from the fresh and dried samples of Bougainvillea spectabilis and Celosia cristata flowers by using different solvents (Aqueous, 50 % methanol, 100 % methanol, 50 % ethanol, 100 % ethanol and acetone). Total betalain (TB), betacyanin (BC) and betaxanthin (BX) content of the flowers were recorded by spectrometric methods. The results revealed that pigment yield was more in Bougainvillea flowers when compared to Celosia in aqueous extraction. On comparing the fresh and dried flowers, dried flowers yielded more betalain content in dried flowers. The extracts of the flowers were screened for antioxidant potentials like ABTS, DPPH, Chelating potential, FRAP and CUPRAC. From the study it was observed that the Bougainvillea and Celosia flowers possess antioxidant potential but the highest was found in the Bougainvillea flower.

Key words : Total betalain, Bougainvillea spectabilis, Celosia cristata, Antioxidant potential.

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

Abstract

                                The haploid induction study is an effective method for inbred and hybrid development quickly. The optimization of media composition for specific indigenous genotypes was a critical factor. Two media namely, Gamborg’s medium (B5) and Murashige and Skoog’s medium (MS) with six different combinations of plant growth regulators were used to evaluate the gynogenic potential of six different genotypes of multiplier onion. Days for embryo induction was earlier in Acc. 1 (46-54), whereas Acc. 4 was the best responding genotype with a higher percent of embryo induction (0.76) followed by Acc. 15 (0.48). The embryo induction rate and survival rate of 0.21% and 54.54% respectively were recorded in B5 medium whereas in MS medium the induction and survival rate was 0.34 % and 34.86 % respectively. Embryo induction ranged from 0 to 1.41% in this study. Five haploids and one mixoploid in B5 medium, five haploids in MS medium were obtained. Ploidy status of the plants was confirmed by flow cytometry, cell cytology, and stomatal dimension analysis.

Key words : Multiplier onion (Allium cepa var. aggregatum), In vitro gynogenesis, Embryo, Haploid, Flow cytometry, Chromosome counting, Stomatal dimension difference.

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

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  • Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

    Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.