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Studies on Extraction of Betalain Pigments by Different Solvents and Assessing Antioxidant Activity of Bougainvillea spectabilis and Celosia argentea Flowers

  • Author:M. Preethi1, N. Sritharan1*, M. Raveendran2 and A. Senthil1

    p-ISSN:0024-9602, Vol:106, Issue:1-3

    DOI:10.29321/MAJ 2019.000230
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Abstract

                              Betalain is a natural pigment with antioxidant property which is used as a food colourant. Betalain was extracted from the fresh and dried samples of Bougainvillea spectabilis and Celosia cristata flowers by using different solvents (Aqueous, 50 % methanol, 100 % methanol, 50 % ethanol, 100 % ethanol and acetone). Total betalain (TB), betacyanin (BC) and betaxanthin (BX) content of the flowers were recorded by spectrometric methods. The results revealed that pigment yield was more in Bougainvillea flowers when compared to Celosia in aqueous extraction. On comparing the fresh and dried flowers, dried flowers yielded more betalain content in dried flowers. The extracts of the flowers were screened for antioxidant potentials like ABTS, DPPH, Chelating potential, FRAP and CUPRAC. From the study it was observed that the Bougainvillea and Celosia flowers possess antioxidant potential but the highest was found in the Bougainvillea flower.

Key words : Total betalain, Bougainvillea spectabilis, Celosia cristata, Antioxidant potential.

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Abstract

                                The haploid induction study is an effective method for inbred and hybrid development quickly. The optimization of media composition for specific indigenous genotypes was a critical factor. Two media namely, Gamborg’s medium (B5) and Murashige and Skoog’s medium (MS) with six different combinations of plant growth regulators were used to evaluate the gynogenic potential of six different genotypes of multiplier onion. Days for embryo induction was earlier in Acc. 1 (46-54), whereas Acc. 4 was the best responding genotype with a higher percent of embryo induction (0.76) followed by Acc. 15 (0.48). The embryo induction rate and survival rate of 0.21% and 54.54% respectively were recorded in B5 medium whereas in MS medium the induction and survival rate was 0.34 % and 34.86 % respectively. Embryo induction ranged from 0 to 1.41% in this study. Five haploids and one mixoploid in B5 medium, five haploids in MS medium were obtained. Ploidy status of the plants was confirmed by flow cytometry, cell cytology, and stomatal dimension analysis.

Key words : Multiplier onion (Allium cepa var. aggregatum), In vitro gynogenesis, Embryo, Haploid, Flow cytometry, Chromosome counting, Stomatal dimension difference.

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For MASU related issues :
SECRETARY
Madras Agricultural Students Union
Tamil Nadu Agricultural University
Coimbatore - 641 003
Tamil Nadu, India.

For MAJ related issues :
CHIEF EDITOR
Madras Agricultural Students Union
Tamil Nadu Agricultural University
Coimbatore - 641 003
Tamil Nadu, India.

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Office: 0422-2436781 , 0422-2966781
Mobile: +91 8300947681

Email: masutnau@gmail.com
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Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

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    MASU has been publishing a reputed journal, “Madras Agricultural Journal” (MAJ) since 1913 and it is one of the longest serving journals in India for the students and research community in agriculture and allied sectors.MAJ has joined the “Directory of Open Access Journal”, Lund, Germany.MAJ are widely cited in research studies

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    • Get In Touch

    For MASU related issues :
    SECRETARY
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    For MAJ related issues :
    CHIEF EDITOR
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    Contact Us

    Office: 0422-2436781 , 0422-2966781
    Mobile: +91 8300947681

    Email: masutnau@gmail.com
    masu@tnau.ac.in , maj@tnau.ac.in

    Social Media

    Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

    Get In Touch

    For MASU related issues :
    SECRETARY
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    For MAJ related issues :
    CHIEF EDITOR
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    Contact Us

    Office: 0422-2436781 , 0422-2966781
    Mobile: +91 8300947681

    Email: masutnau@gmail.com
    masu@tnau.ac.in , maj@tnau.ac.in

    Social Media

    Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.