MADRAS AGRICULTURAL JOURNAL

Effect of Abiotic Factors on the Incidence of Major Pests of Okra (Abelmoschus esculentus) and Natural Enemies

Abstract

                                The present research was aimed to study the seasonal incidence of major sucking pests and the natural enemies on okra Abelmoschus esculentus under field condition at the Department of Agricultural Entomology, Agricultural College and Research Institute, Killikulam. The crop had revealed the incidence of sucking pests viz., aphid, jassid, whitefly, mites along with natural enemies like spiders and coccinellids. The incidence of jassid first commenced in 43rd SMW (0.30/3 leaves), aphids and whitefly in 44th SMW (0.10 and 0.35/3 leaves), mite was found in first SMW (25.53/cm2/leaf area). Earias infestation was found during 49th SMW (7.29 per cent). Natural enemies such coccinellids and spiders were observed during 45th (0.05/ plant) and 43rd SMW (0.05/plant) respectively.

Key words : Aphids, Jassids, Whitefly, Mites, Coccinellids, Spiders.

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

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Abstract

                                Xylitol is a functional sugar alcohol widely used as a sugar substitute. Chemical route of xylitol production is highly expensive, whereas microbial sources offer lower yield and conversion efficiency. In the present investigation, xylose reductase (XR) gene from Candida tropicalis GRA1 was cloned in pQE30Xa vector and transformed into E. coli M15. The resultant recombinant strain produced xylitol from xylose that harboured Xr gene transcribed under the control of lac operon. Such transcribed Xr protein converts xylose into xylitol using NADPH as a co-factor. Xylitol production was achieved by the recombinant E. coli M15 pQE30XrCt under shake-flask fermentation (2.1 g.l-1 in 24 h) with xylose as the sole substrate in the medium. Addition of co-substrate, glucose in the growth medium enhanced the xylitol production to the tune of 3.4g.l-1. Furthermore, the addition of co-substrate glycerol along with xylose enhanced a xylitol yield of 6.4g.l-1

Key words : Candida tropicalis, Xylose Reductase, E. coli M15, Xylose, Xylitol.

Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.

Removal of Lead and Cadmium from Aqueous Solutions by Banana Peel Biochar

Khan Agha Shenwari1, S.Priyatharshini2, P.Dhevagi2, T. Chitdeshwari3 and S. Avudainayagam2    DOI:10.29321/MAJ.2019.000220
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  • Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.