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Efficacy of cry2AX1 Gene in Transgenic Tomato Plants against Helicoverpa armigera and Spodoptera litura

  • Author:J. Sushmitha, E.I. Bamishaiye, N. Balakrishnan, S. Varanavasiappan, L. Arul, S. Mohan Kumar, A. John Joel and D. Sudhakar

    Page No:341

    DOI:10.29321/MAJ.2018.000157
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Abstract

      Tomato crop is infested by large number of insect pests, causing significant yield loss. Fruit borer (Helicoverpa armigera) feeds on fruits which leads to quality detoriation and economic loss. Genetic engineering is recognised as a viable tool to engineer resistance in crops against insect pests. Transgenic tomato plants over-expressing cry2AX1 gene under the control of CaMV35S promoter were developed. The T0 and T1 plants were characterized by PCR for the presence and segregation of transgenes. Segregation of cry2AX1 gene in T1 progeny plants followed the Mendelian pattern of monogenic inheritance of 3:1 ratio. The expression of Cry2AX1 at 55 days after sowing ranged from 0.034 to 0.219 μg/g of fresh leaf tissue and 0.030 to 0.249 μg/g of fresh leaf tissue at 80 days after sowing. Insect bioassay of detached leaf bits performed on the ELISA positive transgenic tomato plants against Helicoverpa armigera and Spodoptera litura resulted in mortality of neonates upto 100 per cent in H. armigera and 53 per cent in S. litura.
Key Words: cry2AX1, Transgenic tomato, Insect resistance, Fruit borer

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-Control (100% RDF), T2 -T1 + Municipal solid waste compost @ 5 t ha-1, T3 -T1 + Municipal solid waste compost @ 10 t ha-1, T4 -T1 + Vermicompost @ 2.5 t ha-1, T5 -T1 + Vermicompost @ 5 t ha-1, T6 -T1 + Rice husk ash @ 5 t ha-1, T6 -T1 + Rice husk ash @ 5 t ha-1, T7 -T1 + Rice husk ash @ 10 t ha-1, T8 -T1 + Lignite Fly ash @ 5 t ha-1, T9 -T1 + Lignite Fly ash @ 10 t ha-1. There were nine treatment combinations replicated thrice in CRD. The soil was sandy in texture pH 7.83 (saline), EC 0.22 dSm-1, available N (257 kg ha-1), available P (10.2 kg ha-1) and available K (117 kg ha-1) respectively which fell in fertility status of low. The soil classified taxonomically as Typic udisamments. The results showed that application of 100% RDF + Vermicompost @ 5 t ha-1 (T5 ) significantly recorded brinjal fruit yield of 934.2 g plant-1 and brinjal stover yield of 403.7g plant-1. The post harvest organic carbon content (3.5 g kg-1), available N (148 mg kg-1) available (7.15 mg kg-1) recorded in the treatment T5 which received 100% RDF + Vermicompost @ 5 t ha-1. The post harvest available K (77.48 mg kg-1) was registered with application of 100% RDF + Lignite Flyash @ 10 t ha-1 (T9 ).

Key words : : Brinjal, Municipal solid waste compost, Vermicompost, Rice husk ash, Lignite flyash. .

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Tamil Nadu Agricultural University
Coimbatore - 641 003
Tamil Nadu, India.

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  • Influence of Different Management Practices and Fertilizer Levels on the Growth and Yield of Rice in Sodic Soil

     K.K. Amrutha and P. Santhy     DOI:10.29321/MAJ.2018.000100
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     Rajesh Jolad, S.D. Sivakumar, C. Babu and N. Sritharan     DOI:10.29321/MAJ.2018.000101
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  • Antifungal Potential of Myco-molecules of Coprinopsis cinerea (Schaeff) S. Gray s.lat. against Fusarium spp

     S. Jeeva and A. S. Krishnamoorthy     DOI:10.29321/MAJ.2018.000102
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  • Active Dispersal of Trichogramma chilonis Ishii and T. pretiosum Riley Towards Host Eggs Under Confined Condition and Their Parasitisation Efficiency

     M. Ranjith, S.J. Nelson and S. Sithanantham     DOI:10.29321/MAJ.2018.000103
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  • Variations in Morphological and Molecular Characterization of Lasiodiplodia theobromae (Pat.) Griffon and Maubl Associated with Coconut Leaf Blight

     P. Ashokkumar, C. Ushamalini and R. Ramjegathesh     DOI:10.29321/MAJ.2018.000104
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  • Development of Essential Oils Based New EC Formulation for the Management of Poist Harvest Anthracnose of Pomegranate Caused by Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.

     M. Sakthi Ishwarya, S. Vanitha, S. Panneerselvam and A. Kamalakannan     DOI:10.29321/MAJ.2018.000105
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  • Persistence and Dissipation Pattern of Dimethoate 30 EC in / on Foxtail Amaranthus and Spinach

     R. Gopalakrishnan, K. Bhuvaneswari, J. Kousika, A. Manivannan and A. Suganthi     DOI:10.29321/MAJ.2018.000106
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  • Demand and Supply Analysis of Roundwood in India

     M. Srinivasan, S. Varadha Raj and A. Eswari     DOI:10.29321/MAJ.2018.000107
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  • Understanding the Value of Tank Ecosystem A Case Study of Tank in Singanallur, Noyyal Basin

     R. Kiruthika and D. Suresh Kumar     DOI:10.29321/MAJ.2018.000108
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  • Dynamics of Land Use Pattern in Kerala – A Temporal Analysis

     B. Sreya and A. Vidhyavathi     DOI:10.29321/MAJ.2018.000109
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  • A Study on Factors Influencing Repurchasing Decision and Constraints Faced by Farmers Towards Mixed Fertilizer in East Godavari District of Andhra Pradesh

     N. Rahul Tagore and K. Divya     DOI:10.29321/MAJ.2018.000110
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  • Cointegration between Domestic and International Market Prices of Beverage Crops in India

     S. Lavanya, U. Arulanandu and S. Selvam     DOI:10.29321/MAJ.2018.000111
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  • Interactive Effect of Plasma Power and Equivalence Ratio upon Plasma Gasification of Coconut Shell

     D. Praveen Kumar, S. Pugalendhi, P. Subramanian, S. Karthikeyan, P. Vijayakumary and J. Gitanjali     DOI:10.29321/MAJ.2018.000112
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  • Experimental Study on Dual Air Supply Downdraft Gasifier for an Improved Producer Gas Quality

     V. Karuppasamy Vikraman, S. Pugalendhi, P. Subramanian, R. Angeeswaran and K. Chandrakumar     DOI:10.29321/MAJ.2018.000113
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  • Thermal Performance Analysis and Prediction of Steam Production from Solar Paraboloid Concentrator using Multiple Linear Regression (MLR)

    Author:P. Komalabharathi, S. Pugalendhi, P. Subramanian, S. Karthikeyan and R. Mahendiran

        DOI:10.29321/MAJ.2018.000114
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  • A Two-Step Catalytic Depolymerization of Alkali Treated Pennisetum glaucum L. and Melia dubia cav. into Low Molecular Weight (LMW) Aromatics

     Nishanth Sekar, Chikunov Andrey, Taran Oxana P, Parmon Valentin N and Sivakumar Uthandi     DOI:10.29321/MAJ.2018.000115
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    • Get In Touch

    For MASU related issues :
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    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

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    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

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    Differential Transcript Expression Profiling of Sugarcane (co 99004) for Elevated Temperature Stress

     R. Gomathi1*, S. Kohila1 and Lakshmi Kasirajan2 DOI:10.29321/MAJ.2018.000169
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    Characterization of Thermo sensitive Red Rice Line for its Suitability in the State of Kerala, India

     R. Gayathri and Roy Stephen DOI:10.29321/MAJ.2018.000170
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    Development of Heat Tolerant Improved White Ponni through Marker Assisted Introgression of QTLs Governing Spikelet Fertility under High Temperature Stress

    D.Vijayalakshmi1*, P.Vivitha1, M.Arumugaperumal1 and M.Raveendran2 DOI:10.29321/MAJ.2018.000171
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    Effect of Elevated Temperature on Physiological Traits andYield Components in Greengram

     N. Sritharan1*, B. Rakavi1, A. Senthil1, P. Jeyakumar1, S. Kokilavani2, S. Pannerselvam2 DOI:10.29321/MAJ.2018.000172
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    Impact of Heat Stress on Physiological traits of Greengram

     Rakavi1* B., N. Sritharan1, A. Senthil1, P. Jeyakumar1, S. Kokilavani2 and S.Pannerselvam2 DOI:10.29321/MAJ.2018.000173
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    Rapid and Nondestructive Methods to Capture the Flower Opening Time in Rice to Identify Donors for High Temperature Stress

    R. Megala1*, R. Priyadharshini1 and D.Vijayalakshmi2 DOI:10.29321/MAJ.2018.000174
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    Differential Responses of Storage Temperature and Ethylene Inhibitors on Postharvest Life of Rasthali Banana

    P. Jeyakumar1*,V. Rajeswari1, M.K. Kalarani2, K.S. Subramanian3 and M. Kavino4 DOI:10.29321/MAJ.2018.000175
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    Effect of Temperature on Shelf life of Muscat Hamburg Grapes under Storage

     U. Tamizheezham*1, I. Muthuvel2 and A. Subbiah3 DOI:10.29321/MAJ.2018.000176
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    Temperature Modifications during Storage to Overcome Weak Neck Disorder in Banana (cv. Rasthali) using α- Amino Isobutyric Acid

     V. Rajeswari1*, P. Jeyakumar1, M.K. Kalarani2, K.S. Subramanian3 and M. Kavino4 DOI:10.29321/MAJ.2018.000177
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    Evaluation of Cassava Genotypes or Salt Tolerance in Nursery

     M.K. Kalarani*, S. Suganya and M. Velmurugan DOI:10.29321/MAJ.2018.000178
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    Effect of Composts and Industrial By-Products on Yield and Postharvest NPK Status of Brinjal under Coastal Saline Soil

    D. Venkatakrishnan1*, G. Sathiyanarayanan2 and D.S. Balaji2 DOI:10.29321/MAJ.2018.000179
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    Heterosis for Yield, Yield components and Sodicity Tolerance Characters in Rice (Oryza sativa L.)

     M. Sureshkumar*1, S.K. Ganesh1 and M. Sakila2 DOI:10.29321/MAJ.2018.000180
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    Impact of Growth Regulating Substances in Improving Crop Establishment and Harvest Index in Blackgram and Greengram under Sodicity

     S. Nithila DOI:10.29321/MAJ.2018.000181
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    Effect of Salt Stress on Antioxidant Enzyme Activities and Nutrient Ion Concentrations in Mango Rootstocks

     P. Deivasigamani1*, R.M. Vijayakumar 1 and R. Sivakumar2 DOI:10.29321/MAJ.2018.000182
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    Effect of Silicon Fertilization on Physiological Parameters and Yield of Hybrid Maize under Irrigated and Rainfed Conditions

     S. Manojkumar1*, R. Karthikeyan1, N. Maragatham1, N.Sritharan2 and K.M. Sellamuthu3 DOI:10.29321/MAJ.2018.000183
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    Get In Touch

    For MASU related issues :
    SECRETARY
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    For MAJ related issues :
    CHIEF EDITOR
    Madras Agricultural Students Union
    Tamil Nadu Agricultural University
    Coimbatore - 641 003
    Tamil Nadu, India.

    Contact Us

    Office: 0422-2436781 , 0422-2966781
    Mobile: +91 8300947681

    Email: masutnau@gmail.com
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    Copyright @ 2019 Madras Agricultural Journal | Developed by Crystalpro All right reserved.