MASU has been publishing a reputed journal, “Madras Agricultural Journal” (MAJ) since 1913 and it is one of the longest serving journals in India for the students and research community in agriculture and allied sectors.MAJ has joined the “Directory of Open Access Journal”, Lund, Germany.MAJ are widely cited in research studies
Tomato crop is infested by large number of insect pests, causing significant yield loss. Fruit
borer (Helicoverpa armigera) feeds on fruits which leads to quality detoriation and economic loss.
Genetic engineering is recognised as a viable tool to engineer resistance in crops against insect
pests. Transgenic tomato plants over-expressing cry2AX1 gene under the control of CaMV35S
promoter were developed. The T0 and T1 plants were characterized by PCR for the presence
and segregation of transgenes. Segregation of cry2AX1 gene in T1 progeny plants followed the
Mendelian pattern of monogenic inheritance of 3:1 ratio. The expression of Cry2AX1 at 55 days
after sowing ranged from 0.034 to 0.219 μg/g of fresh leaf tissue and 0.030 to 0.249 μg/g of fresh
leaf tissue at 80 days after sowing. Insect bioassay of detached leaf bits performed on the ELISA
positive transgenic tomato plants against Helicoverpa armigera and Spodoptera litura resulted
in mortality of neonates upto 100 per cent in H. armigera and 53 per cent in S. litura.
Key Words: cry2AX1, Transgenic tomato, Insect resistance, Fruit borer