MADRAS AGRICULTURAL JOURNAL

Exploiting Biocontrol Potential of Bacillus amyloliquefaciens (sic.) Fukumoto for theManagement of Mungbean Anthracnose

Abstract

                                Experiments were conducted during 2017 to 2018 to manage anthracnose in mungbean caused by Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib using Bacillus spp. A total of eight Bacillus spp were evaluated of which Bacillus amyloliquefaciens (sic.) Fukumoto recorded significantly the highest growth inhibition of 54.8 per cent. The second best antagonist was B. licheniformis with C. lindemuthianum by 48.14 per cent and it was followed by B. endophyticus which inhibited mycelial growth by 41.11 per cent. Bacillus spp tested for the presence of lipopeptide biosynthetic genes viz., Iturin A, Fengycin, and Surfactin, all the eight strains showed positive for fengycin and seven strains were positive for Iturin A and six strains were positive for surfactin. All the three antibiotic genes were found in B. amyloliquefaciens. The crude antibiotics were extracted from B. amyloliquefaciens associated with inhibition of C. lindemuthianum. The presence of antifungal compounds in secondary metabolities were identified by gas chromatography mass spectrophotometry (GC-MS). The strain B. amyloliquefaciens was formulated in liquid for pot culture studies. Seed treatment with B. amyloliquefaciens @ 10 ml/kg seed + foliar spray of B. amyloliquefaciens @ 10ml/ litre at 25 DAS was found to be effective in reducing the incidence of anthracnose in mungbean by 62.05 per cent. This treatment also recorded increased plant height, pods per plant and per plant yield.

Key words : Mungbean anthracnose, Bacillus amyloliquefaciens, Lipopeptide genes, GC-MS